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Nerve & tissue repairEvidence grade: Animal studies only

TB-500

Thymosin beta-4 fragment

A synthetic fragment of thymosin beta-4, studied for cell migration and tissue repair — frequently paired with BPC-157 in repair protocols.

Last reviewed · 1 references

How it works

Thymosin beta-4 is the primary actin-sequestering protein in cells and governs cell migration. TB-500 reproduces its active region. Because repair requires cells to travel to the injury site, promoting migration accelerates several stages of healing simultaneously — alongside reported angiogenic and anti-inflammatory effects.

Animal studies only. Findings come from rodent or in-vitro models. Animal results frequently fail to replicate in people.

What the research reports

  • Accelerated wound healing and reduced scarring in animal models
  • Cardiac repair after infarction in rodents
  • Corneal and dermal healing studies
  • Neuroprotection and improved functional recovery after TBI in rats

What it has not been shown to do

  • No human efficacy trials for musculoskeletal repair
  • Banned by WADA
  • No mental-health data of any kind

Dosing reference

Community protocols commonly cite 2–5 mg twice weekly during a loading phase. Not trial-derived.

Calculate my dose for TB-500
Routes studied
Subcutaneous, Intramuscular
Half-life
Longer-acting than BPC-157, which is why twice-weekly dosing is conventional
Cycle length in the literature
Loading phase of 4–6 weeks, then reduced frequency

Working out a draw volume from a vial? Use the free reconstitution calculator.

Side effects and contraindications

Reported side effects

  • Injection-site reaction
  • Lethargy reported anecdotally
  • Head-rush sensations

Do not use if

  • Active or historic cancer — cell migration promotion is a theoretical metastasis concern
  • Pregnancy and breastfeeding
  • WADA-tested athletes

The practice that shares the mechanism

TB-500 is included for completeness in repair stacks; it has no direct cognitive or mood rationale. Sleep does more for tissue repair than any compound listed here.

What amplifies it

  • Protein sufficiency
  • Deep sleep
  • Progressive tissue loading

Mechanism hubs

Stacks that include it

Common questions

Sources & evidence notes

Evidence grade: Animal studies only1 source · 0 human · 0 animal · reviewed 1 Jun 2026

Findings come from rodent or in-vitro models. Animal results frequently fail to replicate in people.

Confidence score 3/100

Preliminary

Mechanism only. Nothing here establishes that a person would feel anything.

Search all sourcesExports the 1 source currently shown.
  1. 1.

    Annals of the New York Academy of Sciences, 2012 · Source (opens in a new tab)

    In vitroLimited gradeCells or tissue in a dish. Tells you about mechanism, not about outcomes.